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The delicate-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence using a a number of sequence alignment (MSA) offered while in the seqAn library [27]. Wham applies a few filters to the consensus sequences. Breakpoints aren't noted in situations where by consensus sequences are shorter than 10 bp or consist o
Wham clusters each of the prospect breakpoints and rounds their positions to the closest tenth foundation pair. The posture with the best examine assist is reported. Should the smooth-clipped consensus sequence is usually aligned to the putative breakpoint region using the Smith-Waterman algorithm, the breakpoint is further more refined to The situ